Molecular Image Detail

Image

Caption

Generation of the Ercc3^tm1.1Gima allele expressing XPB-YFP^His2HA fused protein. Schematic presentation of the 3' part of the mouse Ercc3 (Xpb) gene locus, including the last exons 14 and 15. The dotted line indicates the 7-kb mouse genomic DNA fragment used for gene targeting. The translational stop codon (in exon 15) was mutated to allow in-frame fusion with the modified fluorescent marker, YFP (i.e., ATG-less YFP, with an additional stretch of six histidines and an HA epitope tags). As a dominant selectable marker, a neomycin cassette (flanked by two LoxP sites) was introduced downstream the YFP sequence.

This image is from Giglia-Mari G, PLoS Biol 2009 Oct;7(10):e1000220, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:154596

Associated
Alleles

Symbol	Name
Ercc3^tm1.1Gima	excision repair cross-complementing rodent repair deficiency, complementation group 3; targeted mutation 1.1, Giuseppina Giglia-Mari

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