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| Caption | (A) Strategy used to generate Sarm1 knockdown mice. To construct the Sarm1 miRNA knockdown transgene, Tg(UBB-EmGFP/RNAi:Sarm1)#Yph, the oligonucleotide cassettes Sarm1i-2045 (5'-TAAGCTTGTCCTCGAATTTGC-3') and Sarm1i-2397 (5'-TAAGGCAGACCCATTGGCGTA-3') were inserted into the vector cDNA6.2-GW/emGFP-miR (Invitrogen). The transgene cassette under the control of the human ubiquitin promoter contains DsREDT3 flanked by two loxP sequences followed by an emGFP cassette. Two artificial miRNAs against mouse Sarm1 were inserted into the 3' end of the emGFP transcript. The transgene cassette also contains insulator. The entire transgene cassette was then dissected from the vector backbone and microinjected into the pronuclei of fertilized C57BL/6J oocytes. Before Cre-mediated recombination, DsREDT3 was expressed and used to identify four red fluorescent founders of the transgenic mice. After crossing with E2A-Cre mice, the DsREDT3 cassette excised itself, resulting in the expression of emGFP-miRNA fusion transcripts. E2A-cre was then washed out by crossing transgenic mice with wild-type C57BL/6J mice. The emGFP signals were used as indicators of artificial miRNA expression. | ||||
| Copyright | This image is from Chen CY, J Cell Biol 2011 May 16;193(4):769-84, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:172355 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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