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| Caption | Generation of Acsl1tm1Rcol allele. A, Acsl1 conditional gene targeting. The targeting construct was designed to delete exon 2 of the gene when crossed to Cre-expressing animals. With excision of exon 2, splicing from exon 1 to downstream exons results in reading frame shifts and immediate translational stops. The positive selection marker (neo) was flanked by frt sites. Both neo and the target region were flanked by loxP sites. The vector also contained the thymidine kinase (TK) gene, allowing for positive-negative selection. B, FlpE/Cre-mediated excisions. Targeted cells were transiently transfected with a FlpE expression vector and screened for the loss of neo by PCR and by cell death when G418 was present in the medium. Targeted neo- cells were microinjected into blastocysts to produce transmitting chimeras. Cre recombinase eliminates exon 2. | ||||
| Copyright | This image is from Li LO, J Biol Chem 2009 Oct 9;284(41):27816-26 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:155714 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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