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| Caption | Generation of the Aspatm1a(EUCOMM)Wtsi and Aspatm1c(EUCOMM)Wtsi alleles. A, Genomic structure of the murine Aspa locus, which spans 6 exons (black boxes). Homologous recombination of the targeting vector inserts the beta-geo cassette (blue box), encoding beta-Galactosidase (lacZ), into intron 1 of the intact Aspa gene. This cassette is flanked by frt-sites (green circles). Additional loxP sites flank exon 2 (red triangles). The vector includes a DTA cassette for negative selection. In the targeted allele, exon 1 is spliced to the splice acceptor site preceding beta-geo, and transcription is terminated at the introduced pA site. The conditional Aspatm1c(EUCOMM)Wtsi (aspaflox) allele is produced by breeding mutants to FLP-deleter mice for recombination of the beta-geo cassette in vivo. Note that frt and loxP sites are not drawn to scale. beta-geo, beta-galactosidase neomycin resistance cassette; SA, splice acceptor; pA, polyadenylation site, DTA, Diphteria toxin gene. | ||||||||
| Copyright | This image is from Mersmann N, PLoS One 2011;6(5):e20336, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:172582 | ||||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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