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| Caption | Generation of the Ly75tm1.1(HBEGF/EGFP)Ksat allele. (A) Strategy used to produce the knock-in mice: (1) Partial restriction map of the wild-type Ly75 (Cd205) gene. Exons are depicted as black filled boxes. The restriction site indicated is X: XbaI. (2) Targeting vector used for the introduction of the mutations in the Ly75 gene. A SalI site engineered in the 3' UTR was used to clone the IRES-DTRGFP-Cre/Neor cassette. Dta, diphtheria toxin a expression cassette; I, internal ribosome entry site; D, DTR; E, EGFP. The Cre/Neor auto-deleter cassette is shown bracketed by Lox P sites (filled triangles); it directs its own excision as it passes through the male germline. (3) Structure of the targeted allele following homologous recombination in recombinant ES clones. (4) Structure of theLy75tm1.1(HBEGF/EGFP)Ksat (Cd205dtr) allele following expression of the Cre recombinase and excision of the Neor cassette in mutant mice. The 3' external single-copy probe (hatched box) and the PCR primers at the 5' end (black arrows) used to verify proper homologous recombination events are shown. | ||||
| Copyright | This image is from Fukaya T, Proc Natl Acad Sci U S A 2012 Jul 10;109(28):11288-93. Copyright 2012 National Academy of Sciences, U.S.A. J:186395 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/17/2024 MGI 6.24 |
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