| Image |
|
||||
| Caption | Generation of the Slc3a2tm1.1Merl allele. (A) Strategy for conditional targeting of the mouse SLC3A2 gene. A-i. Genomic structure of a 31.4 kbp DNA fragment containing the nine exons (numbered red arrow heads), the 5' and the 3' untranslated regions (5'/3'UTR, blue boxes) of the SLC3A2 gene, which encodes CD98. To conditionally knock-out this gene, a loxP (loxP1, green) flanked by HindIII and EcoRV sites was inserted into the NheI site (green, in the 5'UTR). The selection cassette (loxP-TK-neo-loxP) was cloned into the XmnI (green) site downstream of exon 2. To facilitate assembly of the targeting vector, a 7.7 kbp EcoRI and a 7.0 kbp BamHI fragment was sub-cloned into the plasmid pLitmus28. A-ii. Configuration of the targeted CD98 locus. The HindIII and EcoRV sites were used to discriminate between the wild-type and the targeted locus. A-iii. Configuration of the targeted locus after removal of the selection cassette by Cre recombination, after transient expression of Cre recombinase in targeted ES cell clones. "Type II" cells with an excised selection cassette (following Cre recombination between the loxP1 and loxP2 sites) contain an allele which is suitable for conditional gene inactivation by in vivo Cre expression; other recombinatiorial events involving the other loxP site do also occur, a complete deletion yielding a constitutively knocked-out allele often being termed "type I". | ||||
| Copyright | This image is from Nguyen HT, J Clin Invest 2011 May 2;121(5):1733-47 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:173946 | ||||
|
Associated Alleles |
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 12/17/2024 MGI 6.24 |
|
|
|
||
Analysis Tools