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| Caption | Generation of the Cdc25atm1Hpw floxed allele (f) and Cdc25atm1.1Hpw null allele (-). (A) Structure of the targeting vector and chromosomal organization of the Cdc25A locus before and after Cre-mediated excision. The genomic organization of the mouse Cdc25A gene was disrupted by insertion of neomycin phosphotransferase cDNA driven by the phosphoglycerine kinase promoter (pGK-neo) as a selectable marker into intron 3. Exons 1-5 are represented by black boxes. The location of BstXI (B) and XbaI (X) sites are indicated; loxP sites are represented by yellow triangles. The sizes of the upstream (7.5 kb) and downstream (1.2 kb) homologous arms are indicated. The red bars indicate positions of the probes used for Southern blot analysis (internal and 3' probe). The red and blue triangles denote the locations of PCR primers used for genotyping. H, HindIII; Bs, BstE II; +, WT allele; r, recombinant allele; f, floxed allele; -, null allele. | ||||||
| Copyright | This image is from Lee G, Proc Natl Acad Sci U S A 2009 Mar 24;106(12):4701-6. Copyright 2009 National Academy of Sciences, U.S.A. J:145768 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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