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| Caption | Generation of the Igh-Jtm1.1Lave allele. Targeted replacement of the JH cluster with the 2F5 VH gene. Genomic structure of the 2F5 VH targeting construct, the endogenous Ig HC locus, the targeted allele after homologous recombination, and the targeted allele after Cre-mediated neo cassette deletion. The 2F5 VH expression cassette comprises a J558 H10 family VH promoter (p), an H10 split leader sequence (L), and the prerearranged 2F5 VHDJH segment (2F5 VH). Exons are represented as closed boxes, the Igh intronic enhancer (E) is represented by a circle, the 2F5 VH, neo, and CAG-DTA cassettes are represented by shaded boxes, and loxP sites are depicted as triangles. The indicated restriction fragment sizes are indicated for wild-type (WT) and targeted loci. The 5' and 3' probes used to verify homologous recombination events at the 5' and 3' regions of the JH-Eu region, respectively, are shown as black bars. Also indicated are PCR primers to identify (i) homologous recombinant clones (black arrows), (ii) removal of the neo marker (gray arrows), and (iii) germline-transmitted heterozygous (Igh-Jtm1.1Lave/Igh-J+)(2F5 VH+/-) and wild-type (WT) mice (colored arrows). B, Bam HI; RV, EcoRV; n, Nde I. | ||||
| Copyright | This image is from Verkoczy L, Proc Natl Acad Sci U S A 2010 Jan 5;107(1):181-6. Copyright 2010 National Academy of Sciences, U.S.A. J:155798 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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