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| Caption | Generation of the B3gnt5tm1Nari allele. Schematic representation of the murine B3gnt5 targeting strategy. (Top) Wild-type (WT) genomic structure of the mouse B3gnt5 locus is shown. The entire open reading frame (ORF) is in one exon. (Middle) Constructed targeting vector, which contains a replacement neomycin-resistance gene, is shown. (Bottom) Predicted genomic structure of the homologous recombined locus is shown. Restriction enzyme sites indicated are Apa I (A), Ban III (B), Nco I (N), and Sal I (S). Southern blot analysis yielded a 4.8-kb Nco I-digested band corresponding to the WT allele and a 3.4-kb Nco I-digested band corresponding to the targeted (B3gnt5-/-) allele, using an external probe (bold line). Designated primers for genotyping PCR are indicated by arrows. Probe indicates the binding region of the DNA probe used for Southern blot analysis. | ||||
| Copyright | This image is from Togayachi A, Proc Natl Acad Sci U S A 2010 Jun 29;107(26):11900-5. Copyright 2010 National Academy of Sciences, U.S.A. J:161366 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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