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| Caption | Generation of the Del(6Slco1b2-Slco1a5)1Nki allele. (A) To delete all the Slco1a and Slco1b genes, an inverted Pgk-neomycin cassette with loxP sequences was inserted, replacing exons 3 and 4 of Slco1a5. Additionally, a Pgk-thymidine kinase and a Pgk-hygromycin cassette with loxP sequences were inserted, replacing exon 4 of Slco1b2. The Slco1a/1b cluster was subsequently excised from the genome by Pgk-Cre recombinase transfection. Structure of the region after deletion of the Slco1a/1b cluster is shown as well. Probes (5' and 3') used for Southern blot analyses (positioned outside the respective targeting constructs) and forward (F1 and F2) and reverse (R1) primers used for PCR analyses are indicated by lines below the DNA backbone. Gene A and gene B represent Slco1a-like predicted genes EG435927 and EG625716 (according to the NCBI database), respectively. Triangles indicate loxP sequences, numbered boxes indicate exons, and arrows indicate transcriptional orientation. Centr, centromere; Tel, telomere; S, SacI; X, XbaI. | ||||
| Copyright | This image is from van de Steeg E, J Clin Invest 2010 Aug 2;120(8):2942-52 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:163765 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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