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| Caption | Generation of the Nox4tm1.1Hwsc and Nox4tm1.2Hwsc alleles (A) Construct development for Nox4 knockout mice. Exons 14 and 15 are flanked by loxP sites and followed by a floxed neomycin resistance gene (neo) and a negative-selection cassette coding for diphtheria toxin A (dta). Embryonic stem cell clones were generated by homologous recombination with the targeting vector. Transient expression of Cre recombinase results in three different recombination events. Type 1 results in deletion of the neo cassette and thus floxed exons 14 and 15 (Nox4tm1.2Hwsc). These cells can be used to generate conditional Nox4 knockout. Type 2 results in deletion of the floxed exons, and type 3 results in the deletion of exons 14 and 15 and the neo cassette (Nox4tm1.1Hwsc). These cells were used to generate the Nox4 knockout mice. | ||||||
| Copyright | This image is from Kleinschnitz C, PLoS Biol 2010;8(9):, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:166948 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/09/2024 MGI 6.24 |
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