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| Caption | Dact1tm1Yegc/Dact1tm1Yegc mice are perinatal lethal with posterior malformation associated with severe urogenital defects. A: Dact1tm1Yegc/Dact1tm1Yegc (Dact1-/-) mutants are born runt, with a short body and short tail (arrowhead), compared with its wild-type (+/+) and heterozygous (+/-) siblings. B: Double staining of newborn skeletons. E18.5 embryos were stained with Alizarin Red (bone) and Alcian Blue (cartilage). Most of the skeletons are the same between wild-type and mutants, except for the caudal vertebrae (red arrowheads). The mutant exhibited malformed sacral vertebrae and condensed lumbar vertebrae (the region between the red arrows). More than four embryos were examined. C: The urogenital system outline of E17.5 embryos. The mutant (lower panels) showed abnormal fused hydronephrotic kidneys (k), lack of bladder (b), unidentified ureters (ur), misconnected blind-ended uteruses (female, ut) or van deferens (male, vd), compared with heterozygous siblings (upper panels). In contrast, the adrenal glands (a), ovaries (o), and testes (t) of mutant embryos were in normal size and right position. D: Phenotypes of mutant surviving female adult. The mutant 10-month-old female had mild cystic kidneys (k) lack of vagina (*) (left panel), and uterine (ut) hydrometra (upper right panel). The lower right panel showed no vagina open (vg) adjacent to the anal canal (ac). E: H&E stained mid-sagittal section of E13.5 heterozygote (left panel) and homozygous mutant (right panel) sepecimens. The mutant showed a mass of undifferentiated urogenital tissues, instead of developing urogenital tract seen in its heterozygous sibling. ac, anal canal; b, bladder; gt, genital tubercle; uc, umbilical cord; up, urethral plate; udus, undifferentiated urogenital tissues. More than four embyros were examined. F: Shh expression in developing caudal region of wild-type (upper panel) and homozygous mutant (lower panel) embryos at E8.25 (6 somites) (left panel, ventral view), E9 (13 somites) (middle panel, lateral view), and E10.5 (right panel, lateral view), as shown by whole mount in situ hybridzation. Shh mainly expressed in notochord (nc), and gut epithelia, including midgut (mg) and hindgut (hg). The mutant showed truncated and no dilated hindgut and shorter notochord (left and middle panels). At E10.5, no cloaca (Ca) swelled in mutant embryos. More then five mutant embryos have been employed for each stage. st, somites; scale bar, 5 mm in A,B and D, and 0.5 mm in C, E and F. | ||||
| Copyright | This image is from Wen J, J Biol Chem 2010 Apr 2;285(14):11023-30 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:161177 | ||||
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Associated Alleles |
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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