Phenotype Image Detail

Image

Caption

(A) Control, Itgb1^tm1Ref/Itgb1^tm12.1Ref Tg(KRT5-cre)5132Jlj/0 (YY/AA), Itgb1^tm1Ref/Itgb1^tm1Ref Tg(KRT5-cre)5132Jlj/0 (K5-Beta1), and Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0 (hpm) mutant mice at the age of 2 weeks. Itgb1^tm1Ref/Itgb1^tm12.1Ref Tg(KRT5-cre)5132Jlj/0 (YY/AA) and Itgb1^tm1Ref/Itgb1^tm1Ref Tg(KRT5-cre)5132Jlj/0 (K5-Beta1) mice have a reddish skin with a reduced number of hairs and blisters, whereas the hair coat of the Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0 (hpm) mice is only mildly affected. (B) HE-stained paraffin sections of back skin derived from P14 mice show aberrant hair follicle morphogenesis of the skin. The mutant hair follicles fail to grow as deeply into the subcutis as in the littermate control mice and are abnormally shaped. Itgb1^tm1Ref/Itgb1^tm12.1Ref Tg(KRT5-cre)5132Jlj/0 (YY/AA) and Itgb1^tm1Ref/Itgb1^tm1Ref Tg(KRT5-cre)5132Jlj/0 (K5-Beta1) mice have a hyperthickened epidermis and blisters between the epidermis and dermis (arrows). The Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0 (hpm) skin displays an intermediate phenotype. (C) High magnification of the HE-stained back skin section showing blister formation (arrows) and aberrant morphology of the basal keratinocytes in Itgb1^tm1Ref/Itgb1^tm12.1Ref Tg(KRT5-cre)5132Jlj/0 (YY/AA) and Itgb1^tm1Ref/Itgb1^tm1Ref Tg(KRT5-cre)5132Jlj/0 (K5-Beta1) mice. (D) Control, Itgb1^tm1Ref/Itgb1^tm12.1Ref Tg(KRT5-cre)5132Jlj/0 (YY/AA), Itgb1^tm1Ref/Itgb1^tm1Ref Tg(KRT5-cre)5132Jlj/0 (K5-Beta1), and Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0 (hpm) epidermis examined for LacZ activity as a readout of Cre-mediated beta1 integrin gene deletion. LacZ-positive cells are visible in the epidermis and hair follicles of the Itgb1^tm1Ref/Itgb1^tm12.1Ref Tg(KRT5-cre)5132Jlj/0 (YY/AA), Itgb1^tm1Ref/Itgb1^tm12.1Ref Tg(KRT5-cre)5132Jlj/0 (K5-Beta1), and Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0 (hpm) mutant mice.

This image is from Czuchra A, J Cell Biol 2006 Sep 11;174(6):889-99, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:177979

Associated
Alleles

Symbol	Name
Itgb1^tm12.1Ref	integrin beta 1 (fibronectin receptor beta); targeted mutation 12.1, Reinhard Fassler
Itgb1^tm1Ref	integrin beta 1 (fibronectin receptor beta); targeted mutation 1, Reinhard Fassler
Itgb1^tm5.1Ref	integrin beta 1 (fibronectin receptor beta); targeted mutation 5.1, Reinhard Fassler
Tg(KRT5-cre)5132Jlj	transgene insertion 5132, Jose Luis Jorcano

Associated
Genotypes

Allelic Composition	Genetic Background
Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2J
Itgb1^tm1Ref/Itgb1^tm1Ref Tg(KRT5-cre)5132Jlj/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2J
Itgb1^tm1Ref/Itgb1^tm12.1Ref Tg(KRT5-cre)5132Jlj/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2J

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