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| Caption | Pik3c3 is required for secondary fiber cell differentiation and suppression of congenital cataract and microphthalmia. A, Representative dark field images of dissected lens of Pik3c3tm1Tssk/Pik3c3+ Tg(Cryaa-cre)10Mlr/0 (Pik3c3flox/+;MLR10-Cre; control) and Pik3c3tm1Tssk/Pik3c3tm1Tssk Tg(Cryaa-cre)10Mlr/0 (Pik3c3flox/flox;MLR10-Cre; Pik3c3 KO) mice at 0.5 and 7.5 days and 2 months. Scale bar, 1 mm. The equatorial diameter of the lens was quantified (mean +/- S.E.). *,p<0.05; **,p<0.01, unpaired Student's test; n = 6 (0.5 days), 6 (7.5 days), and 4 (2 months). B, Representative images of eyeball and appearance of a 2-month (M)-old mouse. Scale bar, 1 mm. The equatorial diameter of the eyeball at 2 months was quantified (mean +/- S.E.). *,p<0.05, unpaired Student's t test comparing indicated pairs; n = 3. C, Heamtoxylin and eosin staining of the lens of 17.5-dpc control (panels a-c) and Pik3c3 KO (panels d-f) embryos. Magnified images of secondary fiber (panels b and e) and primary fiber cells (panels c and f) in the indicated regions are shown in the right panels. Scale bars, 0.1 mm (panels a and d) and 20 um (panels b, c, e, and f). D, Hematoxylin and eosin staining of the lens from mice at 0.5 and 7.5 days and 2 months. Magnified images in the indicated regions are shown in the right (0.5 and 7.5 days) or lower (2 months) panels. Arrowheads indicate cellular aggregates. Scale bars, 0.1 mm. E, Immunohistochemical analysis of crystallins using anti-beta- and anti-gamma-cyrstallin antibodies in the lens of 0.5-day-old neonates. Scale bar, 100 um. | ||||||
| Copyright | This image is from Morishita H, J Biol Chem 2013 Apr 19;288(16):11436-47 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:198392 | ||||||
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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