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| Caption | The Mesdtm2.1Bch allele does not complement the Mesdtm1Bch (Mesd KO) and Tyrc-3YPSd (albino deletion) alleles. Animals heterozygous for the Cre-recombined Mesdc2 (Mesd-LoxP; Mesdtm2.1Bch) allele were mated to animals heterozygous for the Mesdc2 conventional knockout (Mesd-KO; Mesdtm1Bch) (A-B), the albino (Mesd-3YPSD; Tyrc-3YPSd) deletion (C-D), or the Mesd-LoxP (Mesdtm2.1Bch) allele (E-F). Embryos were dissected at embryonic day (E) 7.5 or E 8.5 and photographed at the indicated magnifications (5X or 2.5X). (A) At E 7.5, gastrulation was near completion in wild-type embryos and the epiblast (ep), amnion (am) and chorion (ch) were clearly visible. (B, B') In contrast, in the Mesdc2 mutant littermate gastrulation was blocked, the extra-embryonic ectoderm (ex) and epiblast (ep) were organized as epithelia, and Reichert's membrane and trophoblast (rm/tb) were expanded in comparison to the epiblast. In panel B', Reichert's membrane was removed to facilitate better visualization of the Mesdc2 egg-cylinder. (C and E) By E 8.5 the size of the wild-type embryo had increased considerably, and the brain (br), heart (he), and somites (so) were clearly visible. (D-F") In contrast, the mutant littermates had only doubled in size, and the epiblast (ep) remained undifferentiated, and Reichert's membrane and trophoblast (rm/tb) were greatly expanded; this layer was removed in panel D' and F'. The embryo shown in panel F' was rotated 90 degrees (F") to allow visualization of the shortened anterior/posterior axis and characteristic indentation on the left side of the pictured Mesdc2 mutant. Previously, we demonstrated that the indentation was coincident with expression of Hex. | ||||||||
| Copyright | This image is from Taibi AV, PLoS One 2013;8(10):e75782, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:209024 | ||||||||
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Associated Alleles |
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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