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| Caption | Ctsltm1Cptr/Ctsltm1Cptr embryos show no alterations in early development of the epiblast but a severe accumulation of vesicles in the yolk sac endoderm. (a) Morphology of Ctsltm1Cptr/Ctsltm1Cptr (-/-) embryos in comparison with heterozygous littermates. The yolk sac of homozygous mutant embryos is less translucent and exhibits an uneven, bulged surface as compared with heterozygous control littermates. (b) Paraffin sections of cathepsin L hetero- and homozygous littermates at E8.0 stained by haematoxylin and eosin (HE). Low magnification (Scale bar, 500 um): the epiblast of the homozygous embryo shows no morphological aberrations. High magnification (Scale bar, 20 um): heterozygous embryos show a normal structure of the visceral endoderm which is characterized by high endocytotic activity indicated by big vesicular structures (*: anterior, **: posterior part). Embryos deficient for cathepsin L exhibit phenotypic abnormalities in the visceral endoderm, such as wrinkled morphology, thickening of the visceral endoderm cell layer, and increased number and size of vesicular structures. (#: anterior, ##: posterior part). (d) Characterization of the vesicles in the visceral endoderm of mutant embryos. Left: the vesicular structures of the visceral endoderm in heterozygous as well as homozygous embryos stain positive for glycoproteins in periodic acid-Schiff (PAS) staining (left panel) as well as for the lysosomal membrane protein LAMP-1 (red) (right panel). The insert shows LAMP-1 vesicles in homozygous mutant embryos at higher magnification. This indicates that the accumulating vesicles are indeed lysosomes. Scale bar, 10 um. | ||||
| Copyright | This image is from Tholen M, Nat Commun 2014;5():4931, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:216506 | ||||
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Associated Alleles |
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/17/2024 MGI 6.24 |
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