Phenotype Image Detail

Image

Caption

Znhit2^em1(IMPC)J/Znhit2^em1(IMPC)J mutants are able to form blastocysts but cannot expand further or hatch out in vitro. Many mutant embryos become collapsed or dead after 24hrs culture. Arrowheads point to scattered DAPI signal, indicating fragmented DNA. Znhit2 mutant embryos exhibit a complete absence of SOX2+ and SOX17+ cells, while NANOG doesnt turn off properly in trophectoderm. RT-PCR results showed the robust expression of Znhit2 and Sox2 in wild-type and heterozygous blastocysts, but not in mutant embryos. BL Mut, mutant blastocyst, CL Mut, collapsed mutant. Additional information at websites.umass.edu/jmager/znhit2

This image was contributed directly to MGI. Questions regarding this image or these data should be directed to Dr. Jesse Mager at jmager@umass.edu J:279207

Associated
Alleles

Symbol	Name
Znhit2^em1(IMPC)J	zinc finger, HIT domain containing 2; endonuclease-mediated mutation 1, Jackson

Associated
Genotypes

Allelic Composition	Genetic Background
Znhit2^em1(IMPC)J/Znhit2^em1(IMPC)J	C57BL/6NJ-Znhit2^em1(IMPC)J/Mmjax

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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