Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  17
• Reference
  J:40771 Kemp SJ, et al., Localization of genes controlling resistance to trypanosomiasis in mice. Nat Genet. 1997 Jun;16(2):194-6
• ID
  MGI:1095682

Genes

Gene	Assay Type
Tir1	visible phenotype
D17Mit135	PCR amplified length variant
D17Mit7	PCR amplified length variant
D17Mit36	PCR amplified length variant
Tir4	visible phenotype
D17Mit28	PCR amplified length variant
D17Mit68	PCR amplified length variant
D17Mit52	PCR amplified length variant

Notes

• Experiment
  A significant variation in the susceptibility of tryponosomiasis in inbred strains of mice enabled the quantitative analysis (QTL) of the trait in genetic crosses. The C57BL/6 strain is relatively resistant compared to the more susceptible strains BALB/cand A/J.
  
  Linkage analysis was performed on F2 mice from both a (BALB/cOlaHsd x C57BL/6JOlaHsd)F2 cross and an (A/JOlaHsd x C57BL/6JOlaHsd)F2 cross. Genotyping was performed on mice with long and those with short survival times selecting for informative microsatellite markers providing coverage of all 19 autosomes and the X chromosome.
  
  Analysis of the (BALB/cOlaHsd x C57BL/6JOlaHsd)F2 cross revealed 3 regions with significant LOD scores.
  
  QTL Tir1 mapped to a region of Chromosome 17 between flanking markers D17Mit135 and D17Mit7 with a LOD score of 11.0 [Fig.1]. Tir1 had an additive effect on survival time and accounted for 14.6% of the variation. D17Mit36 mapped nearest the peak LOD score.
  
  QTL Tir2 mapped to a region of Chromosome 5 between flankingmarkers D5Mit233 and D5Mit10 with a LOD score of 4.6 [Fig.1]. Tir2 also had a significant association with survival time with the resistance allele found to be fully dominant, accounting for 6.7% of the variation. D5Mit58 mapped nearest the peak LOD score.
  
  QTL Tir3 mapped to a region of Chromosome 1 between flanking markers D1Nds2 and D1Mit17 with a LOD score of 5.7 [Fig.1]. This QTL had a additive effect and accounted for 8.9% of the variation. D1Mit425 mapped nearest the peak LOD score.
  
  Analysis of the(A/JOlaHsd x C57BL/6JOlaHsd)F2 cross revealed 2 regions with significant LOD scores on both Chromosome 17 and Chromosome 5. The positions of both QTL appeared to be close to those mapped in (BALB/cOlaHsd x C57BL/6JOlaHsd)F2 cross.
  
  5.27.2015 - Curators Note. Because the second cross here differs from the first we regard the second cross as a separate mapping experiment have have assigned the QTL identified on Chromosomes 17 and 5 with unique identifiers.
  
  In the (A/JOlaHsd x C57BL/6JOlaHsd)F2 cross QTL Tir4 mapped to a region of Chromosome 17 flanked by markers D17Mit28 and D17Mit68 with a LOD score of 9.3 [Fig.1]. Tir4 accounted for 9% of the total variance. D17Mit52 mapped nearest the peak LOD score. The resistance allele was recessive.
  
  In the same cross QTL Tir5 mapped to a region of Chromosme 5 between flanking markers D5Mit255 and D5Mit24 with a LOD score of 3.6 [Fig.1]. Tir5 accounted for 3.9% of the variance and the resistance allele was recessive.
  
  A QTL on Chromosome 1 in the (A/JOlaHsd x C57BL/6JOlaHsd)F2 cross did not acheive significance.