Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  2
• Reference
  J:45910 Perry WL, et al., The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice [see comments]. Nat Genet. 1998 Feb;18(2):143-6
• ID
  MGI:1202369

Genes

Gene	Assay Type	Description
a	reported elsewhere
Itch	direct sequencing	P1-6099

Notes

• Experiment
  In confirming that the a^18H mutation results from an inversion, the authors isolated a series of regional phage P1 clones from mouse Chromosome 2. Sequence analysis of exon-trap products revealed the presence of the Itch gene distal to the agouti locus(a). Both genes transcribe in a proximal to distal direction, relative to centromere. Locus a was found to contain the proximal break in the a^18H mutation, and Itch was found to contain the distal breakpoint