Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  2
• Reference
  J:49152 Qu S, et al., Mutations in mouse Aristaless-like4 cause Strong's luxoid polydactyly. Development. 1998 Jul;125(14):2711-21
• ID
  MGI:1316446

Genes

Gene	Allele	Assay Type
D2Mit221		PCR amplified length variant
D2Mit129		PCR amplified length variant
D2Mit386		PCR amplified length variant
D2Mit351		PCR amplified length variant
D2Mit350		PCR amplified length variant
D2Mit41		PCR amplified length variant
D2Mit439		PCR amplified length variant
D2Mit15		PCR amplified length variant
D2Mit61		PCR amplified length variant
Alx4	Alx4^lst	visible phenotype
D2Mit130		PCR amplified length variant

Notes

• Experiment
  Mutanat progeny of a Stock-lst^Alb x C57BL/6 backcross were genotyped for 62 SSLP markers in order to determine the region of deletion in the lst^Alb chlorambucil mutation. Ten SSLP markers were found to be deleted on mouse Chromosome 2. D2Mit221, D2Mit129, D2Mit386, D2Mit351, D2Mit350, D2Mit41, D2Mit253, D2Mit439, D2Mit15, D2Mit161. To find candidate loci for the mutation, the authors examined the deleted markers in the LSTDP/Fo- + + a/+ + a x (LSTDP/Fo- + + a/+ + a x Fmn^ld-TgHD A/+ + A stock)F1 backcross (see J:35172: Vogt 1996). Three informative markers (D2Mit386, D2Mit439 and D2Mit253) did not recombine with lst, and a fourth, D2Mit161, was placed 0.6 cM distal to lst. Intergration with the JAX BSS backcross panel (C57BL/6JEI x SPRET/Ei)F1 x SPRET/Ei using markers D2Mit14 (proximal to lst), and D2Mit43 (distal to lst) indicated that D2Mit130 does not recombine with lst. Alx4, mapping to the region of D2Mit130, is suggested as a candidate gene for the lst phenotype. ALx4 and lst failed to complement in outcross experiments.