Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  17
• Reference
  J:48214 Samant SA, et al., Mapping and cloning recombinant breakpoints demarcating the hybrid sterility 6-specific sperm tail assembly defect. Mamm Genome. 1999 Feb;10(2):88-94
• ID
  MGI:1331436

Genes

Gene	Assay Type	Description
D17Mit167	overlapping clones
D17Mit45	overlapping clones
D17Mit101	overlapping clones
Pim1	overlapping clones
D17Mit228	overlapping clones
D17Mit229	overlapping clones
D17Mit60	overlapping clones
D17Mit61	overlapping clones
D17Shp1	overlapping clones, Southern analysis	H6.7b
D17Mit134	overlapping clones
D17Mit199	overlapping clones
Ccua	visible phenotype
D17Mit81	overlapping clones
D17Mit135	overlapping clones
Tff3	overlapping clones
Stop1-p	visible phenotype
D17Mit146	overlapping clones
D17Mit191	overlapping clones
Ccub	visible phenotype
Cryaa	overlapping clones
D17Mit175	overlapping clones
D17Mit63	overlapping clones
D17Leh89
138017-Sp6
138017-T7
173G10-Sp6
173G10-T7
188G13-Sp6
188G13-T7
203J18-Sp6
203J18-T7
220G15-Sp6
220G15-T7
272E3-Sp6
272E3-T7
283H8-T7
293E15-Sp6
293E15-T7
317A21-Sp6
317A21-T7
324M10-Sp6
324M10-T7
338D4-Sp6
338D4-T7
351P6-T7
371M4-T7
37I12-Sp6
37I12-T7
393F19-Sp6
393F19-T7
503G1-Sp6
503G1-T7
51J6-Sp6
51J6-T7
566K2-Sp6
566K2-T7
58205-Sp6
58205-T7
58C20-SP6
58C20-T7
613H23-Sp6
613H23-T7

Notes

• Reference
  The authors in J:107013 (Hui L; Lu J; Han Y; Pilder SH) in 2006 show that the original Ccub can be divided into two separate genetic elements one (Ccub1) mapping between Dnahc8 and D17Mit191 and the other (Ccub2) mapping with Pou5f1. Tsga2 was suggested as a strong candidate for Ccub1
• Experiment
  The authors examined the expression of the Hst6-specific flagellar assembly phenotype in sperm from mice homozygous for M. spretus - M. m. domesticus recombinant Chromosome 17 homologs whose breakpoints map within the hst6 locus. SSLP analysis of these homologs demonstrate that the flagellar assembly defect, "whipless" maps to a region less than 0.2 cM between D17Mit61 and D17Mit135, coincident with Ccua. These studies also defined more precisely the map positions of the Ccub and Stop1-p genes. The proximal two thirds of the Stop1-p locus has been cloned as part of the Tff3 BAC contig and covers ~465kb. The T-2p BAC carries the proximal breakpoint for the Stop1-p locus and the distal breakpoint of the Ccua locus. The proximal breakpoint of the Ccua locushas been isolated on the H-A BACa clone. The Stop1-p distal/Ccub proximal recombinant breakpoint has not isolated. Analysis of 23 BACs mapping to four contigs within the Hst6 locus gave a probable order of the markers used in this study on mouse Chromosome 17 as: D17Mit167 - D17Mit45 - D17Mit101 - Pim1 - (D17Mit228, D17Mit229, D17Mit60, D17Mit61) - D17Shp1 - (D17Mit134 - D17Mit199) - D17Mit81 - D17Mit135 - Tff3 - D17Mit146 - D17Mit191 - Cryaa, D17Mit175) - D17Mit63 - D17Leh89. The order of the markers within the brackets has not been determined. The additional markers here represent the BAC end clones used to determine the physical orientation of the loci wihin the contig..