Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  11
• Reference
  J:53010 Guler ML, et al., Tpm1, a locus controlling IL-12 responsiveness, acts by a cell-autonomous mechanism. J Immunol. 1999 Feb 1;162(3):1339-47
• ID
  MGI:1332573

Genes

Gene	Assay Type
D11Mit314
Tcpm1	visible phenotype
Il5

Notes

• Experiment
  The authors used 4 recombinant animals from a previous backcross (BC1) to generate congenic animals (BC2) for each crossover on a BALB/c background. These mice (BC2) were generated by mating BC1 mice to BALB/c DO-TCR +/+ transgenic mice. The BC2 mice were assayed for the Tcpm1 phenotype. The assay test for the ability of in vitro stimulated T cells to maintain responsiveness to Il12. BALB/c mice lose Il12 responsiveness while B10.D2 and (B10.D2 x BALB/c)F1 mice maintain Il12 responsiveness. Based on the analyis of the phenotype and the recombination event in each line, the authors were able to localize the QTL, Tcpm1 to a 0.45 cM interval between D11Mit314 and Il5 on mouse Chromosome 11. This analysis rules out Il5, Csfgm and Irf1 as candidate genes. Il4and Il13 and other genes centromeric to Il5 are possible candidates.