Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  19
• Reference
  J:51890 Cox GA, et al., Identification of the mouse neuromuscular degeneration gene and mapping of a second site suppressor allele. Neuron. 1998 Dec;21(6):1327-37
• ID
  MGI:1333392

Genes

Gene	Allele	Assay Type
Igk-Vt		overlapping clones
Cox6a3		overlapping clones
Ighmbp2	Ighmbp2^nmd-2J	visible phenotype
D19Mit32		overlapping clones
Cpt1a		overlapping clones
Gal		overlapping clones
Cdk2ap1		overlapping clones
Ptprcap		overlapping clones

Notes

• Experiment
  To fine map nmd (Ighmbp2^nmd-2J), an initial (B6.BLKS-nmd^2J x CAST/EI)F2 mapping cross that localized nmd to Chromosome 19 (J:23584, Cook, 1995) was expanded by genotyping and examining the phenotype of 1524 interspecific F2 progeny representing 3048 independent meiotic events. The segregation of the recessive impaired locomotor phenotype was examined, and proximal Chromosome 19 markers generated from overlapping YAC, BAC, and candidate cDNA's were examined for evidence of recombination around the nmdlocus. D19Mit32 was found to be inseparable from nmd as was Ighmbp2, indicating these markers are within a common 200kb genomic region. Expression studies with nmd^2J and nmd^J strains supported the identification of Smbp2 as the gene involved with thenmd phenotype. The presented physical map shows the following overall order of loci on mouse Chromosome 19: Igk-Vt - Cox6a-ps - Ighmbp2 - D19Mit32 - 5' - Cpt1a - 3' - Gal - Doc1 - Ptprcap.