Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  19
• Reference
  J:54055 Ji W, et al., Identification of genes within the Krd deletion on mouse chromosome 19. Mamm Genome. 1999 Apr;10(4):399-401
• ID
  MGI:1334737

Genes

Gene	Assay Type	Description
Scd1	visible phenotype
Nkx2-3	Southern analysis	Nkx2-3 probe
Wnt8b	Southern analysis	Wnt8b probe 2
Fgf8	Southern analysis	Fgf8 probe 7
Abcc2	SSCP	Cmoat-pA, Cmoat-pB
Tlx1	Southern analysis	Tlx1 probe 2
Krd	visible phenotype

Notes

• Experiment
  The authors analyzed progeny from the (C57BL/6J-Krd/+ x SPRET/Ei) cross to determine the genes located within the Krd deletion on mouse Chromosome 19. Failure to transmit a C3H or C57BL/6J allele along with the Krd transgene indicated deletion of the locus. The authors used Southern analysis and SSCP to detect the alleles. Using this approach, Fgf8, Wnt8b, Tlx1, Nkx2-3 and Cmoat were localized to the Krd deletion. The authors also analyzed progeny form the (ab/ab x C57BL/6J-Krd/+) cross. All of the Krd/+mice showed signs of asebia, indicating that the ab gene is also located within the Krd deletion.