Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  5
• Reference
  J:52453 Sharpe J, et al., Identification of sonic hedgehog as a candidate gene responsible for the polydactylous mouse mutant Sasquatch. Curr Biol. 1999 Jan 28;9(2):97-100
• ID
  MGI:1338383

Genes

Gene	Assay Type
Ssq	visible phenotype

Notes

• Experiment
  FISH analysis revealed the presence of two integration sites for the transgene on mouse Chromosome 5. Further, the Msx1 and 1x markers were shown to lie between the two integration sites. Further analysis indicated that the proximal intergration site co-localized with the Shh gene. Ssq was backcrossed with M. m. castaneus to determine which of the intergration sites localized with Ssq. Only mice homozygous for the proximal insertion site displayed the homozygous Ssq phenotype. FISH analysis suggested that the proximal site was at a distance of ~ 800kb relative to the Ssh gene. The authors suggest that the Ssq phenotype is a result of interference with the cis regulation of the Shh gene.