Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  4
• Reference
  J:54987 Vogel SN, et al., Cutting edge: functional characterization of the effect of the C3H/HeJ defect in mice that lack an Lpsn gene: in vivo evidence for a dominant negative mutation. J Immunol. 1999 May 15;162(10):5666-70
• ID
  MGI:1342475

Genes

Gene
D4Mcg10
D4Nds9
D4Mcg8
D4Mit25
363o12-T7
3f1
Trim32
3b11
277i15-T7
256f23-T7
3a4
277i15-SP6
1h9
D4Mcg6
D4Rck137
2b9
D4Mcg28
D4Mcg27
D4Mcg21
D4Mcg16
D4Mcg24
D4Mcg26
D4Mcg15
D4Mcg18
D4Mcg25
D4Mcg22
Tlr4
D4Mcg5
D4Mit178
D4Mcg47
D4Mcg54
D4Mcg49

Notes

• Experiment
  The authors carried out high resolution mapping of the Tlr4 region on mouse Chromosome 4. The authors used (C3H/Rl x 101/RL)F1 mice carrying deletions around the Tlr4 locus. These mice are maintained in a heterozygotic state with a normal, "balancer" copy of Chromosome 4 to insure viability. The Tyrp1^b-11R30M strain carried a deletion which eliminated the Tlr4 region while the Tyrp1^b-13R75M starin carried an equivalently large deletion distal to the Tlr4 region. Tyrp1^b/Tyrp1^b-11R30M mice as well as Tyrp1^b/Tyrp1^b-13R75M mice were crossed with C3H/HeJ mice which are hyporesponsive to LPS. F1 progeny from these crosses were genotyped for markers that distinguished "balancer" Chromosome 4 from the copy of Chromosome that contained the deletion, as well as C3H/HeJ Chromosome 4 markers. Informative markers used in the analysis were ordered along the chromosome. The order of markers on mouse Chromosome 4 is: D4Mcg10 - D4Nds9 - D4Mcg8 - D4Mit25 - 363o12-T7 - 3f1 - Trim32 - 3b11 - 277i15-T7 - 256f23-T7 -3a4 - 277i15-SP6 - 1h9 - D4Mcg6 - D4Rck137 - 2b9 - D4Mcg28 - D4Mcg27 - D4Mcg21 - D4Mcg16 - D4Mcg24 - D4Mcg26 - D4Mcg15 - D4Mcg18 - D4Mcg25 - D4Mcg22 - Tlr4 - D4Mcg5 - D4Mit178. D4Mcg47, D4Mcg49 and D4Mcg54 are located between 256f23-T7 and D4Mcg6. All of the typed markers including Tlr4 were deleted in the Tyrp1^b-11R30M strain but not in the Tyrp1^b-13R75M strain.