Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  5
• Reference
  J:57318 Saigoh K, et al., Intragenic deletion in the gene encoding ubiquitin carboxy-terminal hydrolase in gad mice [see comments]. Nat Genet. 1999 Sep;23(1):47-51
• ID
  MGI:1345321

Genes

Gene	Allele	Assay Type
D5Mit197
Uchl1	Uchl1^gad	visible phenotype
D5Mit110
D5Mit151

Notes

• Experiment
  Authors used an intersubspecific backcross between MSM and DBA/2J mice to map D15Mit151 distal to D15Mit197. Subsequent studiees using BAC and YAC clones revealed that D5Mit110 was mapped between D5Mit197 and D5Mit151 and linked to the gad gene in the backcross panel between gad and C57BL/6J mice. using D5Mit110 as a probe, sveral BACs were cloned from a BAC library. Exon trapping and subsequent computer-based analyses revealed the presence of sequences similar to human and rat Uchl1. A full length mouseUchl1 cDNA clone was isolated. PCR analysis of Uchl1 cDNA revealed alteration in genomic organization in gad mice. A 3.5kb segment including exons 7 and 8 of the Uchl1 gene was deleted in gad mice. Western blot analysis provided further evidence that this alteration in the Uchl1 gene caused the mutation in gad mice.