Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  2
• Reference
  J:57571 Ikeda A, et al., Genetic modification of hearing in tubby mice: evidence for the existence of a major gene (moth1) which protects tubby mice from hearing loss. Hum Mol Genet. 1999 Sep;8(9):1761-7
• ID
  MGI:1346654

Genes

Gene	Allele	Assay Type
Map1a	Map1a^+	visible phenotype
D2Mit17

Notes

• Experiment
  A mutation in the tub gene leads to obesity and retinal and cochlear degeneration. QTL analysis for auditory brainstem response (ABR) thresholds was carried out with (AKR/J x C57BL/6J-tub/tub)F2 intercross mice to identify modifers affecting hearing ability. The marker D2Mit17 was highly associated with click ABR thresholds (P = less than 4.22 chi square 10e(-34), LOD = 33.4). The AKR/J allele of this QTL normalized ABR thresholds in tubby mice in a dominant fashion; a single copy prevented hearing loss. This QTL, designated as Mtap1a was responsible for 57% of the click threshold variance observed in this intercross. The presence of this QTL was confirmed by ABR analysis on F1 hybrids construted from a C57BL/6J stock (C57BL/6J.129-B2m^tm1/unc congenic for a 129/Ola derived donor segment including Mtap1a. The 129/Ola congenic segment on Chromosome 2 extened from D2Mit442 to D2Mit107 and covered the majority of the 95% confidence interval identified for moth1 in the AKR intercross. The C57BL/6J-tub/tub.moth1^129/Ola/moth1^B6 mice showed normal ABR thresholds for all sound simulations