Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  6
• Reference
  J:60691 Hovland DN, et al., Identification of a murine locus conveying susceptibility to cadmium-induced forelimb malformations. Genomics. 2000 Jan 15;63(2):193-201
• ID
  MGI:1858272

Genes

Gene	Assay Type
Cadfar	resistance/susceptibility
D6Mit390

Notes

• Experiment
  The heavy metal cadmium (Cd) is a potent teratogen in mice and induces forelimb defects in the C57BL/6 strain while no limb defects are observed in identically treated SWV strain. To identify potential fetal loci influencing differential sensitivity to cadmium-induced forelimb malformations, a series of QTL analysis was performed among 198 [C57BL/6 x (C57BL/6 x SWV)]F1 backcross mice utilizing a linkage map of 149 microsatellite markers. A penetrance scan of the binary trait detected one locus with a lodscore of 2.67 (P = 0.00045) located on the distal end of mouse Chromosome 6 near D6Mit390. The binary data were converted to a semi-quantitative trait based on the severity of the defect and analyzed by the parametric and non-parametric scan functions of MAPMAKER/QTL. Both analysis identified the same QTL on Chromosome 6, just centromeric of D6Mit390. The peak lod score was 2.32 when analyzed with the non-parametric approach and 3.08 when analyzed with the parametric scan. This locus has been designated as Cadfar.