Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  9
• Reference
  J:59437 Eden PA, et al., Biochemical and immunogenetic analysis of an immunodominant peptide (B6dom1) encoded by the classical H7 minor histocompatibility locus. J Immunol. 1999 Apr 15;162(8):4502-10
• ID
  MGI:1859010

Genes

Gene	Assay Type
D9Mit182
H7	PCR amplified length variant, immunological
D9Mit18

Notes

• Experiment
  The authors used CTL-mediated immunoselection to map the gene encoding B6-dom1 to mouse Chromosome 9. Refined mapping using loss of heterozygosity (LOH) analysis of the variant cultures mapped the gene telomeric to D9Mit182. this map position was confirmed by analyzing the strain distribution patterns (SDPs) of B6-dom1 in inbred strains. The SDP was most consistent with the telomeric region of Chromosome 9 near D9Mit18. Allele typing of markers on distal Chromosome 9 in two congenic strains B10.C-H7^c (47N) and B6.C-H7,c> (HW23) showed that the region predicted to contain the B6-dom1 locus is of BALB/c origin in 47N mice but of B6 origin in HW23 mice. Functional analysis of CTLs in both congenic strains suggested that B6-dom1 is encoded by the classical H7 locus. Using the genetic and functional studies, the authors suggest that the HW23 strain defines a second minor H locus (referred to as H61) which is distinct from and maps proximal to H7.