Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  9
• Reference
  J:63231 Wilson SM, et al., A mutation in Rab27a causes the vesicle transport defects observed in ashen mice. Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7933-8
• ID
  MGI:1861719

Genes

Gene	Allele	Assay Type
D9Mit73
D9Mit75
D9Mit210
Rab27a	Rab27a^ash	visible phenotype
D9Mit340
D9Mit166
D9Mit341
D9Mit342
D9Mit156
D9Mit305

Notes

• Experiment
  The authors used 897 [(C3H/heSn-ash/ash x M. castaneus) X C3H/heSn-ash/ash] N2 progeny to define an ash critical region of 600kb on mouse Chromosome 9. The order/distance of markers is: D9Mit73, D9Mit75, D9Mit210 - 0.1 +/- 0.1 cM - Rab27a, D9Mit340, D9Mit341 - 0.2 +/- 0.2 cM - D9Mit342, D9Mit156 - 0.3 +/- 0.2 cM - D9Mit305. The Rab27a gene was identified as a candidate in this region. Analysis of the gene revealed a single A - to - T transversion in a splice donor site in mutant mice. This would lead to the production of truncated proteins. BAC rescue experiments confirmed that Rab27a ebcoded ash.