Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  15
• Reference
  J:63420 Ho AM, et al., Role of the mouse ank gene in control of tissue calcification and arthritis [see comments]. Science. 2000 Jul 14;289(5477):265-70
• ID
  MGI:1888563

Genes

Gene	Assay Type
D15Mit251
Ank	visible phenotype
D15Mit20
D15Mit164
D15Mit155

Notes

• Experiment
  The authors used 923 F2 ank/ank mice from a cross with M. castanneus to map the ank locus to proximal mouse Chromosome 15. D15Mit20 and D15Mit164 did not recombine with the ank trait, placing them at less than 0.6 cM from the mutation. Using D15Mit20 as an entry point, the authors constructed a ~400kb BAC contig spanning the ank locus. End probes from BACs 104F8 and 144P21 were mapped on opposite sides of ank in the high-resolution mapping cross, showed that the ank mutation was in the cloned region. In vivo complementation studies with BAC transgenic mice showed that BAC 28M13 restored wild type phenotype to ank/ank mice. Sequencing studies with the BAC identified a nonsense mutation in an ORF on the BAC. This sequence change was not found in 17 wild type inbred strains suggesting that it was the ank mutation. Subsequent functional studies confirmed that this was the ank mutation.