Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  5
• Reference
  J:63394 Clark RM, et al., A novel candidate gene for mouse and human preaxial polydactyly with altered expression in limbs of Hemimelic extra-toes mutant mice. Genomics. 2000 Jul 1;67(1):19-27
• ID
  MGI:1888948

Genes

Gene	Assay Type
D5Kng2	overlapping clones
Rnf32	overlapping clones
Lmbr1	overlapping clones
Hm
Shh
D5Kng3	overlapping clones
Nom1	overlapping clones
Mnx1	overlapping clones

Notes

• Experiment
  The authors used a high resoution genetic cross, chromosome walking experiments and BAC sequencing to define a candidate interval of 295-570kb containing the Hm mutation. The order of markers around this interval were: D5Kng2 - Rnf32 - Lmbr1 - D5Kng3 - D5Kng1 - Hlxb9. The Lmbr1 and Lmbr2 genes maps within the critical interval defined for the mouse and human limb mutations. Expression studies have also shown misregulation of Lmbr1 gene expression in Hx mutant mice. Previous studies have shown tight linkage between the Hx and Hm mutations.