Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  X
• Reference
  J:66695 Brunkow ME, et al., Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat Genet. 2001 Jan;27(1):68-73
• ID
  MGI:1929911

Genes

Gene	Allele	Assay Type
DXCch1		overlapping clones
DXMit55		overlapping clones
AA273854		overlapping clones
Ppp1r3f		overlapping clones
Foxp3	Foxp3^sf	overlapping clones
Ccdc22		overlapping clones
Cacna1f		overlapping clones
DXMit26		overlapping clones
Syp		overlapping clones
Prickle3		overlapping clones
Plp2		overlapping clones
Magix		overlapping clones
Gpkow		overlapping clones
Ppia-ps1		overlapping clones
Wdr45		overlapping clones
Praf2		overlapping clones
Ccdc120		overlapping clones
Smt3h2-ps		overlapping clones
Tfe3		overlapping clones
DXCch4		overlapping clones
Gripap1		overlapping clones
Kcnd1		overlapping clones
Otud5		overlapping clones
DXCch2		overlapping clones
Pim2		overlapping clones
DXCch5

Notes

• Experiment
  Authors used an intersubspecific backcross to localize sf to a 0.3 cM interval on Chromosome X. A BAC contig was constructed across the interval and 4 BACs 196K10, 34916, 8C22 and 284K10 defined a minimal tiling path over the estimated 500kb region. Computational analysis of the sequence from this region corresponded well with a transcript map generated in J: 62168. The order and orientation of markers in this region was determined to be: centromere - DXCch1 - DXMit55 - AA273854 - 3' - Ppp1r3f - 5' - 5'-Foxp3 - 3' - 3' - DXImx40e - 5' - 5' - Cacna1f - 3' - DXMit26 - 5' - Syp - 3' - 5' - Lmo6 - 3' - 3' - Plp2 - 5' - 3' - Pdzx - 5' - 5' - DXImx41e - 3' - 5' - Ppia-ps - 3' - 5' - Wdr45 - 3' - 5' - DXImx39e - 3' - 3' - AA030924 - 5' - 5' - Smt3h2-ps - 3'- (5' - Tcfe3 - 3' - DXCch4) - 5' - Gripap1 - 3' - 5' - Kcnd1 - 3' - 5' - Otud5 - 3' - DXCch2 - 5' - Pim2 - 3'. Candidates for the sf mutation were analyzed by direct sequencing of RT-PCR products from mutant and normal animals. A frameshift resulting in atruncated gene product was observed in the Foxp3 gene of sf animals. The identity of the Foxp3 gene was confirmed by the use of transgenic animals.
  
  DXCch5 also mapped to mouse Chr. X was was not shown in the contig.