Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  7
• Reference
  J:65815 Engemann S, et al., Sequence and functional comparison in the beckwith-wiedemann region: implications for a novel imprinting centre and extended imprinting. Hum Mol Genet. 2000 Nov 1;9(18):2691-706
• ID
  MGI:1930526

Genes

Gene	Assay Type
Mrpl23	overlapping clones, direct sequencing
Nctc1	overlapping clones, direct sequencing
H19	overlapping clones, direct sequencing
Igf2	overlapping clones, direct sequencing
Igf2os	overlapping clones, direct sequencing
Ins2	overlapping clones, direct sequencing
Th	overlapping clones, direct sequencing
Ascl2	overlapping clones, direct sequencing
Tspan32	overlapping clones, direct sequencing
Cd81	overlapping clones, direct sequencing
Tssc4	overlapping clones, direct sequencing
Trpm5	overlapping clones, direct sequencing
Kcnq1	overlapping clones, direct sequencing
Kcnq1ot1	overlapping clones, direct sequencing
Cdkn1c	overlapping clones, direct sequencing
Slc22a18	overlapping clones, direct sequencing
Phlda2	overlapping clones, direct sequencing
Nap1l4	overlapping clones, direct sequencing
Cars1	overlapping clones, direct sequencing
Tnfrsf23	overlapping clones, direct sequencing
Tnfrsf22	overlapping clones, direct sequencing
Osbpl5	overlapping clones, direct sequencing

Notes

• Experiment
  Based on a contig of BAC clones covering a 1 Mb imprinting cluster region, the authors sequenced a region on mouse Chromosome 7 that is homologous with the Beckwith-Wiedemann syndrome (BWS) region on human Chromosome 11p15.5. A combination of homology searches and sequence analysis tools were used to order and orient genes in this region. The order was: centromere - 5' - Mrpl23 - 3' - Nctc1 - 3' - H19 - 5' - 3' - Igf2 - 5' - 5' - Igf2as - 3' - 3' - Ins2 - 5' - Th - 3' - Ascl2 - 5' - 5' - Tspan32 - 3' - 5' - Cd81 - 3' - 5' - Tssc4 - 3' - 3' - Trpm5 - 5' - 5' - Kcnq1 - 3' - Kcnq1ot1 - 5' - Kcnq1 - 3' - 3' - Cdkn1c - 5' - 5' - Tssc3 - 3' - Slc22a1l - 5' - 3' - Nap1l4 - 5' - 3' - Cars - 5' - 3' - Tnfrsf1al1 - 5' - 3' - Tnfrsf1al2 - 5' - 3' - Osbp2 - 5'.