Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  1
• Reference
  J:66211 Dragani TA, et al., Mapping of melanoma modifier loci in RET transgenic mice. Jpn J Cancer Res. 2000 Nov;91(11):1142-7
• ID
  MGI:1931472

Genes

Gene	Assay Type
Melm1	visible phenotype
D1Mit494	PCR amplified length variant
D1Mit150	PCR amplified length variant
Cxcr4
Inhbb
Ifi201
Ifi202a
Ifi203
Ifi204
H25
Itpkb
Terf1
Traf5
Melm2	visible phenotype
Tgfb2

Notes

• Experiment
  Line 304 is a RET transgene that was maintained in a (C57BL/6 x BALB/c)F1 background. The RET transgenic line is a model for melanoma development. A partial congenic line was developed to N3 by crossing (C57BL/6 x BALB/c)F1 transgenic carriers to C57BL/6J. Importantly these N3 congenics were heterozygous for the RET transgene. The N3 RET congenics were crossed to BALB/c and then backcrossed to the N3 RET transgenic line producing 195 backcross mice for the purpose to scan the genome for melanoma phenotypes. Two markers on mouse Chromosome 1 were associated with the early onset of melanoma. The first of these, Melm1 was associated significantly with D1Mit494 (LOD 2.5), with a second marker Melm2 significantly associated with marker D1Mit150 (LOD 4.2). Authors suggest Cxcr4 and Inhbb as candidates for Melm1 and Ifi201, Ifi202a, Ifi203, Ifi204, H25, Itpkb, Tgfb2, Terf1 and Traf5 as candidates for Melm2. Another marker, Melm3 was associated with mouse Chromosome 11.