Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  4
• Reference
  J:68682 Li X, et al., High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal Chromosome 4. Mamm Genome. 2001 Jan;12(1):13-6
• ID
  MGI:1933951

Genes

Gene	Assay Type
D4Mit256	PCR
Pusl1	PCR
Tas1r3	visible phenotype
Tas1r1

Notes

• Experiment
  A total number of 629 F2 chromosomes were typed using a DNA mapping panel from an F2 intercross between 129P3/J and C57BL/6ByJ strains. The animals were produced from reciprocal outcrossing of 129P3/J females and C57BL/6ByJ males, or C57BL/6ByJ females and 129P3/J males. This panel was informative to map Tas1r3 as a QTL to a 2.6 cM region flanked by proximal marker D4Mit256 and distal marker D18346. It was also noted that Tas1r3 mapped distal of Gpr70.
  
  The authors provided the following to MGI via an email submissions.
  
  Phenotyping: Consumption of 120 mM sucrose and 17 mM saccharin was measured in individually caged mice using 96-h two-bottle tests, with water as the second choice. Fluid intakes were expressed per 30 g of body weight (BW, the approximate weight of an adult mouse) per day, or as a preference score (ratio of average daily solution intake to total fluid intake, in percent).
  Mapping: LOD scores were estimated under the free model. Peak LOD scores for both sucrose (LOD 19.7) and saccharin (LOD 16.0) intakes are located in the interval D4Mit256 - D18346, 1.3 cM distal to D4Mit256 and 1.3 cM proximal to D18346. This locus explained 14.0% and 15.4% of phenotypical variance of sucroseand saccharin intakes respectively. The confidence intervals (LOD drops of 1.0) extend 0.2 cM (sucrose) or 0.6 cM (saccharin) proximally to D4Mit256, and distally beyond D18346 (the distal boundary could not be estimated in cM because no markers distal to D18346 have been genotyped). The B6 allele was dominant over the 129 allele because the peak LOD scores estimated under the free model were within 1 LOD unit from those estimated under B6 dominant model, whereas they were more than 1 LOD unit higher than those estimated under additive or B6 recessive models. Analysis of preference scores showed similar results.