Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  16
• Reference
  J:68109 Sinha D, et al., A temperature-sensitive mutation of crygs in the murine opj cataract. J Biol Chem. 2001 Mar 23;276(12):9308-15
• ID
  MGI:1934633

Genes

Gene	Allele	Assay Type
Crygs	Crygs^Opj	PCR amplified length variant, direct sequencing

Notes

• Experiment
  Since the Opj mutation and the Crygs gene had been mapped to Chromosome 16, the authors analyzed the sequence of the Crygs gene in Opj mutant mice. A single base change that replaced a phenylalanine to serine was identified in the N-terminal domain. Biochemical studies showed that the mutant protein has decreased solubility and associated loss of secondary structure at physiological temperatures.