Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  1
• Reference
  J:68417 Hitzemann R, et al., Effect of genetic cross on the detection of quantitative trait loci and a novel approach to mapping QTLs. Pharmacol Biochem Behav. 2000 Dec;67(4):767-72
• ID
  MGI:2137484

Genes

Gene
Bslm3
Hpic2
Etlm1

Notes

• Experiment
  The authors use a novel approach in mapping QTLs involved in murine behavioral phenotypes. A genome wide scan was performed with (C57BL/6J x DBA/2J)F2 and (BALB/cJ x LP/J)F2 intercrosses crosses to map QTLs affecting in three different behavioral phenotypes. The strains chosen have widely differing polymorphisms and phenotypes, and the QTLs identified from each cross do not appear to have overlap. Significant QTLs (LOD>4.3 threshold) for basal locomotor activity (Bslm3), haloperidol-induced catalepsy (Hpic2), and ethanol-induced locomotion (Etlm1) were mapped to Chromosome 1 at positions 55 - 85 cM, 70 - 100 cM, and 70 - 100 cM, respectively. Etlm1 and Hpic2 were identified from the (C57BL/6J x DBA/2J)F2 cross. Bslm3 was identified from the (BALB/cJ x LP/J)F2 cross. The authors also used a subtractive method to resolve a broad QTL (60 - 100 cM) for basal activity on distal Chromosome 1. Genotyping information from (C57BL/6J x DBA/2J)F2, (C57BL/6J x A/J)F2, and (C57BL/6J x BALB/cJ)F2 intercrosses was used to screen 254 microsatellite markers across the locus. Only markers polymorphic between all four strains (35 markers) were further analyzed. These highly informative markers revealed 2 separate QTLs: one previously reported by Talbot for basal activity at 67 cM and another at 95 - 105 cM.