Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  1
• Reference
  J:54322 Suto J, et al., Quantitative trait loci that regulate plasma lipid concentration in hereditary obese KK and KK-Ay mice. Biochim Biophys Acta. 1999 Mar 30;1453(3):385-95
• ID
  MGI:2137603

Genes

Gene	Assay Type
Cq1	visible phenotype
Cq2	visible phenotype
D1Mit425	PCR amplified length variant
D1Mit150	PCR amplified length variant

Notes

• Experiment
  95 microsatellite markers at an average density of 14.4-16.5 cM were used to map loci affecting obesity-associated plasma lipid concentrations in 190 (C57BL/6J x KK-Ay)F2 animals. KK-Ay animals develop severe obesity and elevated plasma lipid concentrations while C57BL/6J remain lean with lower lipid levels. F2 animals of genotype a/a at the agouti locus have an intermediate plasma cholesterol phenotype while F2 animals of genotype Ay/a have a plasma cholesterol phenotype resembling the KK-Ay parental strain.
  
  Three QTLs involved in plasma cholesterol concentrations were identified: Cq1 (LOD = 4.6) and Cq2 (LOD = 5) are linked to D1Mit425 and D1Mit150, respectively, on mouse chromosome 1, and Cq3 (LOD = 4.3) is linked to D3Mit102 on chromosome 3.
  
  Cq2 in linkage with D1Mit150 maps to within 1.1 cM of the Apoa2 locus. Sequencing of Apoa2 cDNA in C57BL/6J and KK revealed 7 nucleotide substitutions (resulting in 3 amino acid substitutions) within the open reading frame.
  
  Nq1 (LOD = 4.3), a QTL involved in plasma NEFA (non-esterified fatty acids) concentrations, mapped to chromosome 9 in association with D9Mit353.