Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  12
• Reference
  J:61343 Ishizaki K, et al., Fine mapping of smallest common regions of deletion on chromosome 12 in liver epithelial and hepatocellular carcinoma cell lines from B6C3F(1) and C3B6F(1) mice. Int J Cancer. 2000 Apr 15;86(2):251-4
• ID
  MGI:2148132

Genes

Gene	Assay Type
Scrd1	Deletion Mapping
Scrd2	Deletion Mapping
D12Mit217
D12Mit158
D12Mit263
D12Mit79
D12Mit20

Notes

• Experiment
  44 liver epithelial and hepatic carcinoma cell lines derived from (C57BL/6J x C3H/HeJ)F1 and (C3H/HeJ x C57BL/6J)F1 mice were screened for loss of chromosomal regions associated with cell line establishment. Loss of heterozygosity (LOH) at Chromosome 12(43.8% LOH at D12Mit217, D12Mit158, and D12Mit263) and 4 (56.3% LOH at D4Mit13; 53.1% at D4Mit308) were prevalent. Preferential loss of C57BL/6J alleles on Chromosome 12 and 4 (P<0.01) appears to be associated with cell line establishment in both crosses. The locus on Chromosome 12 was resolved to 2 distinct loci, Scrd1 at 6 cM and Scrd2 to the 5 cM range between D12Mit79 (53 cM) and D12Mit20 (58 cM). Scrd1 has synteny to human Chromosome 2p while Scrd2 has synteny with human Chromosome 14q32. LOH in thesehuman chromosomal regions has been reported in various cancers. Other loci also observed to have LOH coincident with cell line establishment map to Chromosome 1 (12.5%), Chromosome 8 (12.5%), Chromosome 11 (15.6%), and Chromosome 14 (21.9%).