Mapping Data

Experiment

• Experiment
  TEXT-Meta Analysis
• Chromosome
  1
• Reference
  J:69668 Turri MG, et al., QTL analysis identifies multiple behavioral dimensions in ethological tests of anxiety in laboratory mice. Curr Biol. 2001 May 15;11(10):725-34
• ID
  MGI:2148624

Genes

Gene	Assay Type
Axtex	visible phenotype
Axtofd7	visible phenotype
Axtofd8	visible phenotype
Axtpmd	visible phenotype
Axtldbd	visible phenotype
Axtldbd1	visible phenotype
Axtmcd	visible phenotype

Notes

• Reference
  DeFries strains:
  QTL were mapped in two large F2 intercrosses from four lines derived from a replicated selection experiment for open-field activity, an animal model for susceptibility to anxiety. The foundation for the selection experiment consisted of an F3 generation derived from a cross between inbred lines, BALBc/J and C57BL/6J. Ten F3 litters that contained at least two males and females were chosen at random. The most active male and female within each litter were selected to become the progenitors of one selected line, termed H1. The least active male and female from each of the same ten litters were selected to serve as progenitors of a low activity line, L1. The same selection procedure was applied to members of a second randomly chosen group of 10 F3 litters as parents for replicate selected lines H2 and L2. The resulting progeny represented the first selected generation. Within the two high lines the most active male and female were selected from each litter, whereas the least active male and female were selected from each litter in the two low lines.Thirty generations of selection were completed and in generation 30 open field activity scores of the high lines were 30 times greater than those of the low lines.
  Following the suspension of selection the lines were randomly mated within line for 18 generations and then inbred using brother-sister mating.
  
• Experiment
  A large set of ethological measures of anxiety-related behaviors were analyzed to explore the relationship between the behavioral phenotype and genotype of anxiety. Phenotypic and genotypic data from a total of 1636 mice from two intercosses of (H1 x L1)F2 and (H2 x L2) F2 DeFries strains decribed in a separate paper (J:70479) were obtained.
  
  Five behavorial tests were conducted in the open field arena, the elevated plus maze, the square maze, the light dark box and the mirror chamber. The test results from the two crosses were highly consistent and to maximize power and mapping resoultion the data was combined from both crosses in the current companion study. Likely QTL positions were determined using MAPMAKER-QTL and QTL-CARTOGRAPHER.
  
  The results of this study mapped 3 major QTL influencing anxiety-related behaviors on Chromosomes 1, 4 an 15. Other anxiety-related behavioral QTLs with multiple effects mapped to Chromosomes 7, 12, 14, 18 and X.
  
  The major QTL mapping to Chromosome 1, Axtex, anxiety-exploratory behavior, had an influence on a broad reange of measures in both anxiogenic and nonanxiogenic compartments.
  
  The combined LOD scores from both crosses, for each signficant test measurement contibuting to Axtex follow:
  anxiety-exploratory behavior, open field, total activity, LOD=27.4;
  anxiety-exploratory behavior, open field, center activity, LOD=14.8;
  anxiety-exploratory behavior, open field, center time, LOD=3.9;
  anxiety-exploratory behavior, elevated plus maze, closed arm entries, LOD=9.4;
  anxiety-exploratory behavior, elevated plus maze, closed arm activity, LOD=6.7;
  anxiety-exploratory behavior, elevated plus maze, closed arm time, LOD=4.3;
  anxiety-exploratory behavior, elevated plus maze, open arm entries, LOD=13.7;
  anxiety-exploratory behavior, elevated plus maze, open arm activity, LOD=21.1;
  anxiety-exploratory behavior, elevated plus maze, open arm time, LOD=3.4;
  anxiety-exploratory behavior, square maze, closed arm activity, LOD=21.3;
  anxiety-exploratory behavior, square maze, open arms activity, LOD=11.3;
  anxiety-exploratory behavior, square maze, open arm entries, LOD=9.2;
  anxiety-exploratory behavior, light dark box, light box time, LOD=13.0;
  anxiety-exploratory behavior, light dark box, light box activity, LOD=24.8;
  anxiety-exploratory behavior, light dark box, dark box activity, LOD=7.4;
  anxiety-exploratory behavior, light dark box, transitions, LOD=16.7;
  anxiety-exploratory behavior, light dark box, latency to emerge LOD=4.9.
  
  At each locus the direction of effect of the allele was examined from the less active strains, L1 and L2. All traits measured at Axtex were decreased by the influence of the L1 or L2 allele with the exception of latency to emerge in the light dark box test which was increased by the influence of the L1 or L2 allele. [Table 2].
  
  Table 3 gives the mapping results for loci influencing defecation in each test apparatus. Differentiation of defecation scores by components of the test apparatus were not possible. However results were consistent for each apparatus.
  
  The following QTL influencing defecation in the test apparatus were mapped to Chromosome 1, LOD scores from combined cross data:
  
  Axtofd7, anxiety-open field defecation 7, open field day 1, mapped to 74 cM, LOD=14.50.
  Axtofd8, anxiety-open field defecation 8, open field day 2, mapped to 74 cM, LOD=13.25.
  Axtpmd, anxiety-plus maze, defecation mapped to 72 cM, LOD=7.41.
  Axtldbd, anxiety-light dark box defecation, day 1 mapped to 84 cM, LOD=5.59.
  Axtldbd1, anxiety-light dark box defecation 1, day 3 mapped to 84 cM, LOD=6.63.
  Axtmcd, anxiety-mirror chamber defecation, LOD=3.33.
  
  All Chr 1 defecation QTL were influenced by the L1 or L2 allele which increased defecation.