Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  4
• Reference
  J:52834 Allen RD, et al., Genetics of graft-versus-host disease, I. A locus on chromosome 1 influences development of acute graft-versus-host disease in a major histocompatibility complex mismatched murine model. Immunology. 1999 Feb;96(2):254-61
• ID
  MGI:2149637

Genes

Gene	Assay Type
Gvhd2	visible phenotype
D4Mit42
Tnfrsf8	reported elsewhere

Notes

• Experiment
  64 polymorphic markers were typed in a population of (B10.D2-H2^d x DBA/2J)F1 x B10.D2-H2^d backcross mice and (B10.D2-H2^d x DBA/2J)F1 x DBA/2J backcross mice to identify QTLs associated with graft-versus-host disease (B10=C57BL/10J; D2=DBA/2J). Spleen cell from backcross animals were intraperitoneally injected into (C57BL/10J x DBA/2J)F1 recipients to induce graft-versus-host disease, and splenomegaly was measured as an indicator of disease. F1 animals receiving spleen cells from (B10.D2-H2^d x DBA/2J)F1 x B10.D2-H2^d backcross donors showed higher incidence of graft-versus-host disease than F1 animals receiving spleen cells from (B10.D2-H2^d x DBA/2J)F1 x DBA/2J backcross donors. Donor animals homozygous for C57BL/10J-derived alleles appear to induce more severe disease in F1 recipients. QTL analysis revealed a major locus on distal mouse chromosome 1 segregating with this trait. This locus, Gvhd1 (graft-versus-host disease 1), maps to 78 cM - 112 cM on mouse chromosome 1 yielding a peak LOD score = 27 at D1Mit150. Candidate genes near the region of Gvhd1 are Cd48 and Mtv7. A second locus, Gvhd2, mapped to approximately 76 cM - 81 cM on mouse chromosome 4 yielding a peak LOD score = 7.2 near D4Mit42. A candidate gene in the region of Gvhd2 is Tnfrsf8 (Cd30). Animals homozygous for C57BL/10J-derived alleles at both Gvhd1 and Gvhd2 induced the most severe disease response in F1 recipients. A suggestive locus mapped to mouse chromosome 6 near D6Mit86 (P<0.02).