Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  4
• Reference
  J:65671 Araujo LM, et al., NKT lymphocyte ontogeny and function are impaired in low antibody-producer Biozzi mice: gene mapping in the interval-specific congenic strains raised for immunomodulatory genes. Int Immunol. 2000 Nov;12(11):1613-22
• ID
  MGI:2149902

Genes

Gene	Assay Type
Im2	visible phenotype
D4Mit31
D4Mit40
Tnfsf8	reported elsewhere
Jak1	reported elsewhere
Bmfr1	reported elsewhere
Txlna	reported elsewhere
Csf3r	reported elsewhere
Ifna	reported elsewhere

Notes

• Experiment
  Im2 (immunoregulatory 2), a QTL on mouse chromosome 4 affecting antibody response, was one of several immunoregulatory loci mapped in 1996 by Puel et al (J:37521). Interval specific congenic strains (ISCS) were constructed for each immunoregulatory QTL on mouse Chromosome 4, 6, 8, 10, 12, and 18. Animals from a high (H) antibody responder line were successively backcrossed to the low (L) antibody responder line to fix H-derived alleles for each QTL on an L-derived background. Each ISCS line was challenged with i.v. injection of anti-CD3 mAb and analyzed for IL4 response. The ISCS line carrying Im2, the H-derived Chromosome 4 QTL spanning 51 cM - 59 cM, retained a significant high responder phenotype (P<0.05), thus confirming the locus. Candidate genes inthe region of Im2 include Tnfsf8 (Cd301), Jak1, Bmfr1, Il14, Csf3r, Idd11, and Ifna. Sle2 is a QTL associated with autoimmune disease in the region of Im2.