Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  5
• Reference
  J:65696 Koyner J, et al., Identification and time dependence of quantitative trait loci for basal locomotor activity in the BXD recombinant inbred series and a B6D2 F2 intercross. Behav Genet. 2000 May;30(3):159-70
• ID
  MGI:2150035

Genes

Gene	Assay Type
Actd2	visible phenotype
D5Mit72
Actd3	visible phenotype
D5Mit278

Notes

• Experiment
  A 2-tiered approach was used to identify QTLs associated with basal locomotor activity in rodents, as measured by distance traveled in an open field test during a 20 minute time interval. First, 25 BXD recombinant inbred (RI) strains were typed for 400 polymorphic markers at an average spacing of 4 cM to identify potential QTLs (B = C57BL/6J, D = DBA/2J). Second, potential QTLs were confirmed by screening approximately 500 animals from a (C57BL/6J x DBA/2J)F2 intercross. Parental strain C57BL/6J exhibits more activity compared to parental strain DBA/2J. A locus on mouse chromosome 1, Actd1, was significantly associated with locomotor activity in both the RI strains and (C57BL/6J x DBA/2J)F2 intercross. Actd1 maps to approximately 65 cM (LOD=10.5) on mouse Chromosome 1 with a QTL range of 40 cM - 90 cM. QTLs on mouse chromosome 5, Actd2 at 9 cM (P<0.001 at D5Mit72) and Actd3 at 61 cM (P<5 x 0.0001 at D5Mit278), were identified and confirmed, and a QTL on mouse Chromosome 9, Actd4 at 28 cM (P<2 x 0.0001 at D9Mit94), was identified and confirmed. C57BL/6J-derived alleles increase locomotor activity at Actd1, Actd2, and Actd3, while DBA/2J-derived alleles increase locomotor activity at Actd4.