Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  13
• Reference
  J:67850 Valenza-Schaerly P, et al., A dominant modifier of transgene methylation is mapped by qtl analysis to mouse chromosome 13. Genome Res. 2001 Mar;11(3):382-8
• ID
  MGI:2150761

Genes

Gene	Assay Type
Modm	visible phenotype
D13Mit21
D13Mit97
D13Mit64
D13Mit126
D13Mit202

Notes

• Experiment
  QTL mapping was performed on a cross between (BALB/cJ x C57BL/6J)F1 females and E36 transgenic C57BL/6J males. The hepatitis B transgene is differentially methylated depending upon parent of origin. Paternal transmission to a C57BL/6J background results in undermethylation while paternal transmission to a BALB/cJ background results in hypermethylation. Maternal transmission to any genetic background results in methylation. 102 microsatellite markers at an average spacing of 11 cM was typed in 88 (BALB/cJx C57BL/6J)F1 x E36 progeny for QTL mapping. A major locus, Modm (modifier of methylation), was detected on mouse Chromosome 13 spanning 30 cM - 45 cM with a maximum LOD = 16.5 near D13Mit21 and D13Mit97. This locus includes the site of the transgene insertion. Modm was confirmed in 4 CXB (C = BALB/cByJ, B = C57BL/6ByJ) recombinant inbred (RI) strains. CXB RI strains homozygous for BALB/cByJ-derived alleles at Modm exhibited 80%-60% methylation at the transgene locus whereas CXB RI strains homozygous for C57BL/6ByJ-derived alleles exhibit 30%-20% methylation at the transgene locus.