Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  4
• Reference
  J:68139 Casley WL, et al., Detection of quantitative trait loci affecting caffeine metabolism by interval mapping in a genome-wide scan of C3H/HeJ x APN F(2) mice. Drug Metab Dispos. 1999 Dec;27(12):1375-80
• ID
  MGI:2150831

Genes

Gene	Assay Type
Cafq2	visible phenotype
D4Mit199
D4Mit17
D4Mit26
D4Mit166
D4Mit255
D4Mit155

Notes

• Experiment
  QTL mapping was performed on 438 F2 animals from an intercross between the acetaminophen nonsusceptible (APN) strain and C3H/HeJ to identify loci associated with caffeine metabolism. The average spacing between markers is 13 cM. Caffeine metabolism QTLs mapped to mouse Chromosome 1 (Cafq1), mouse Chromosome 4 (Cafq2), and mouse Chromosome 9 (Cafq3). Cafq1 is located 85 cM - 95.8 cM on mouse Chromosome 1 with a peak LOD = 9.7 at D1Mit356. No obvious candidate genes are found near Cafq1. Males show a stronger association to the phenotype than females at this locus. Cafq2 is located 31.4 cM - 57 cM on mouse Chromosome 4 with a peak LOD = 3.4 at D4Mit199. Cafq3 is located 28 cM - 48 cM on mouse Chromosome 9 with a peak LOD = 25 at D9Mit248 and D9Mit21. Cyp1a2 is a likely positional candidate gene for Cafq3 based on its known role in caffeine metabolism and differences in protein and mRNA expression of Cyp1a2 between the parental strains.