Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  4
• Reference
  J:71658 Cool M, et al., Elevated frequency of loss of heterozygosity in mammary tumors arising in mouse mammary tumor virus/neu transgenic mice. Cancer Res. 1999 May 15;59(10):2438-44
• ID
  MGI:2151792

Genes

Gene	Assay Type
Naad1	Deletion Mapping
D4Mit236
Naad2	Deletion Mapping
D4Mit111
Naad3	Deletion Mapping
D4Mit178
Naad4	Deletion Mapping
D4Mit12
Naad5	Deletion Mapping
D4Mit54
Cdkn2a	reported elsewhere
Cdkn2b	reported elsewhere
Pla2g2a	reported elsewhere
Lci	reported elsewhere

Notes

• Experiment
  86 microsatellite markers at an average spacing of 20 cM (approximately 74% genome coverage) were screened in 62 mammary carcinomas from (BALB/c x C57BL/6)F1 MMTV/neu trangenic female mice to identify loci involved in mammary tumor formation. Several loci designated Naad (Neu associated allelic deletion) were identified on mouse Chromosomes 4, 19, and 8 in association with loss of heterozygosity (LOH) and/or allelic imbalance (AI). On mouse Chromosome 4, Naad1 is associated with 28% LOH at D4Mit236 (12.1cM), Naad2 is associate with 32% LOH at D4Mit111 (21.9 cM), Naad3 is associated with 42 % LOH at D4Mit178 (35.5 cM), Naad4 is associated with 37% LOH at D4Mit12 (57.6 cM), and Naad5 is associated with 58% LOH at D4Mit54 (66 cM). Preferential loss of BALB/c alleles is observed at these loci, Naad1-Naad5 (21/30 mammary tumors). Naad3 maps near 2 candidate tumor suppressor genes, Cdkn2a and Cdkn2b, and colocalizes with a thymic lymphoma suppression QTL, Tlsr1 (Santos et al, J:31797). Naad4 maps near Lci (liver cell immortalization-Lee et al, J:30263) at 57.5 cM. Naad5 maps near possible candidate gene Plag2a (Mom1) and colocalizes with a thymic lymphoma suppression QTL, Tlsr2 (Santos et al, J:31797). On mouse Chromosome 19, Naad6 is associated with 24%-26% LOH at D19Mit19 and D19Mit10 (26 cM - 47 cM) and Naad7 is associated with 26% LOH at D19Mit34 (53 cM). Preferential loss of C57BL/6 alleles is observed at Naad6 and Naad7 (15/20 mammary tumors). Naad6 maps near Pten (phosphatase and tensin homolog) and colocalizes with Pas3, a pulomonary adenoma susceptibility QTL. On mouse Chromosome 8, Naad8 is associated with 18% LOH at D8Mit86 (52 cM) and maps near the E-cadherin gene, Cdh1. Naad9 is associated with 21% LOH at D8Mit91 (67 cM) and maps near candidate tumor suppressor genes Aprt and Cdh15.