Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  11
• Reference
  J:55284 Ueda H, et al., Genetic analysis of late-onset type 2 diabetes in a mouse model of human complex trait. Diabetes. 1999 May;48(5):1168-74
• ID
  MGI:2156505

Genes

Gene	Assay Type
Nidd1n	visible phenotype
Nidd4n	visible phenotype
D11Mit236
D11Mit195
D11Mit76
Hnf1b	reported elsewhere

Notes

• Experiment
  Genome scan was performed on 42 animals from a (C3H/HeNCrlCrlj x NSY/NcuOs)F2 intercross to identify QTLs associated with type 2 diabetes phenotypes. Parental strain NSY is non-obese and develops insulin resistance and impaired insulin response in an age-dependent manner leading to development of type 2 diabetes.
  
  Significant linkage to diabetes phenotypes was detected on mouse Chromosomes 6 (Nidd3n), 11 (Nidd1n and Nidd4n), and 14 (Nidd2n). Nidd1n maps to 20 cM - 47 cM on mouse Chromosome 11 between D11Mit236 and D11Mit95 and is associated with glucose tolerance (LOD=9.5), blood glucose levels at all ages (LOD=8.49), and fasting blood glucose levels (LOD=4.33) at 36 weeks of age. NSY-derived alleles confer impaired glucose tolerance in a dominant or additive fashion. A possible candidate gene for Nidd1n is Tcf2. Sequence analysis revealed a threonine to alanine (T222A) allelic variation between strains NSY/NcuOs and C3H/HeNCrlCrlj in the DNA binding domain of Tcf2.
  
  Nidd2n maps to 15 cM - 40 cM on mouse Chromosome 14 between D14Mit160 and D14Mit59 and is associated with glucose tolerance after 24 weeks of age (LOD=4.88) and basal insulin levels (LOD=4.52). Nidd2n does not affect glucose tolerance in early life (12 weeks). NSY-derived alleles confer impaired glucose tolerance in a recessive or additive fashion at Nidd2n. Nidd3n maps to 32.5 cM - 38.5 cM on mouse Chromosome 6 between D6Mit209 and D6Mit178 and is associated with epididymal fat weight (Efw) (LOD=6.75), basal insulin levels (LOD=4.69), blood glucose levels at 36 weeks (LOD=3.51), and insulin/glucose ratio (LOD=3.2). Linkage to glucose levels at 36 weeks of age peaked near D6Mit52 (appx. 69.4 cM).
  
  Nidd3n affects glucose tolerance after 24 weeks of age but not in early life (12 weeks). NSY-derived alleles confer impaired glucose tolerance in a dominant fashion at Nidd3n. Nidd4n maps to 2 cM on mouse Chromosome 11 near D11Mit76 and is associated with glucose tolerance (LOD=5.79) in early life (12 weeks) and insulin/glucose ratio (LOD=5.35).NSY-derived alleles confer impaired glucose tolerance in a recessive or additive fashion at Nidd4n.
  
  Nidd1n, Nidd2n, and Nidd3n show overlap with previously mapped type 1 diabetes susceptibility QTLs Idd4, Idd12, and Idd6, respectively.
  
  Efw mapped to mouse Chromosome 6 within a region including D6Mit209 and D6Mit178.