Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:76791 Henckaerts E, et al., Genetically determined variation in the number of phenotypically defined hematopoietic progenitor and stem cells and in their response to early-acting cytokines. Blood. 2002 Jun 1;99(11):3947-54
• ID
  MGI:2387297

Genes

Gene	Assay Type
Hrec	visible phenotype
D2Mit495	PCR amplified length variant
D2Mit411	PCR amplified length variant
Jag1	reported elsewhere
Bmp2	reported elsewhere
Il1a	reported elsewhere
Il1b	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 28 C57BL/6J x DBA/2J (BXD) recominant inbred (RI) strains to identify loci associated with hematopoietic response to early cytokines. Primitive progenitor cells of parental strain C57BL/6J exhibit stronger proliferation in response to flt3L, KL, and TPO compared to parental strain DBA/2J. Also, the absolute number of Lin-Sca1++ cells is 2-fold higher in C57BL/6J than in DBA/2J. A major locus, Hrec, mapped from 73.2 cM (D2Mit495) - 77.6 (D2Mit411) cM on mouse Chromosome 2with LRS = 18.8 for proliferative response and LRS = 9.4 for absolute number of Lin-Sca1++ cells. C57BL/6J-derived alleles confer increased proliferation and number of Lin-Sca1++ cells at Hrec whereas DBA/2J-derived alleles confer decreased proliferation and cell numbers. This locus was confirmed in congenic strain B6.D2-(D2Mit133-D2Mit200) which carries a DBA/2J-derived segment of chromosome 2 DNA from 66.8 cM - 107 cM on a C57BL/6J genetic background. This congenic exhibits significantly fewer Lin-Sca1++ cells compared to the C57BL/6J background strain thus confirming the decreasing effect of DBA/2J-derived alleles at Hrec. Possible candidate genes for Hrec are Jag1, Bmp2, Il1a, and Il1b. A highly suggestive QTL linked to Lin-Sca1++ cell number mapped to approximately 79 cM on mouse Chromosome 4 (D4Mit33 and D4Mit343) with LRS = 15.9. DBA/2J-derived alleles confer increased cell numbers at this locus.